The gopher Sensor is a compact pH meter that measures pH potentiometrically using a pH and reference electrode.  The sensor can also measure temperature and humidity.

2.1 The sensor should not be submersed in any liquid.  The sensor is water resistant. Submersing the sensor completely into a liquid can result in premament damage.

2.2 The sensor should be used with a filter when measung protein enriched liquids.  Protein may contaminate the probe and will reduce the longevity of the sensor.

2.3 The sensor well should be soaked with Buffer A solution for a minimum of 4 hours and preferably 12 hours before starting any measurement cycle.

Refer to gopher elite user guide for instructions about connecting the sensor to the elite;

https://gopherivf.com/gopher-elite-user-guide/

Refer to the Connecting the Bridge to the WIFI User Guide on how to connect sensors to the Bridge

Connecting Sensors to the Bridge

Refer to the User Interfae Guide

6.1 The gopHer is simple to calibrate and will require the pH buffer kit (PN1234).

6.2 On the software, select the sensor and select calibrate pH.

6.3 Follow the instructions as displayed on the software. Rinse the Sensor well with DI water and then with 2 x Buffer A solution. Place approx. 1 mL Buffer A solution in the well and press Next and wait until further instructions appear on the screen.

6.4 Once the Buffer A solution is measured, the gopHer will ask you to rinse with 2 x DI water and then 2 x Buffer B solution. Place approx. 1 mL Buffer B solution in the well and press

6.5 Next, after the Buffer B has been measured, rinse the well with 2 x DI water and then rinse the well with 2 x Quality Check Solution. Place approx. 1 mL of Quality Check Solution into the well and press Next

6.6 After this step, the software will either pass the calibration or ask you to repeat the calibration.

7.1 Using a paif of tweezers, remove a filter from the filter pack.  Do not use the blue separators between the filters.

7.2  Do not touch the filters with your hands, use gloves if necessary.

7.1 Use the gopher filter press to place a filter into the sensor.   Place a filter on to the filter guide, placed the filter press onto the sensor and then press down onto the plunger.

7.4 With the filter press still on the sensor, lift of the plunger and remove the filter press from the filter.

7.5 You will be left a formed filter that fits into the sensor as shown below.

9.1 Prepare the sensor for running media by ensuring the gopher well has a media inside the well

9.2 Pipette 800- 1000ul of Meida into the well.

9.3 Use the silicone lid or approx 8 drops of oil to prevent the evaporation of media.

9.4 The Sensor will instantly start reading the pH and send the information to the monitor or the bridge.  Place the sensor into an incubator that is close to the monitor or the bridge.

Maintenance of the sensor will prolong the life of the sensor.

After the sensor is run with media it is strongly advisable to follow the cleaning process and let the sensor rest in Buffer A solution for a minimum 4 hours and preferably overnight before using the sensor again.

10.1.1 The sensor should be cleaned after being used .

10.1.2  Rinse the probe in warm tap water (around 60°C / 140°F).

10.1.3 Scrub the sensor well with a soft goat hair brush with the mild detergent or enzyme cleaner for one minute. After scrubbing rinse with warm water and then with deionized water.

CAUTION – Never brush the well without rinsing and flushing thoroughly with water (tap water, demi-water etc).  Debris and particles may be on the sensor surface and brushing them into the sensor may damage it.  When in doubt, soak the probe for a while in warm water with a mild detergent.

10.1.4 It is recomended to use Pepsin-HCl cleaning solution (i.e. Mettler Toledo Material No. 51350100) to clean the sensor after using protein infused media.  Place approx 1 mL of the Pepsin HCl cleaning solution into the well and let it sit for 1 hour or longer.  After soaking the well with Pepsin-HCl solution, rinse the well with distilled water.

10.1.5 Dry the well with a soft cloth or a kimwipe.  Soak the sensor in Buffer A.  Place a lid on the well to avoid evaporation of the buffer

10.1.6 WAIT A MINIMUM OF 4 HOURS with Buffer A solution in the well before using the sensor again.  The sensor MUST be calibrated prior to using it again.

10.2.1 Revitializing is required when the probe cannot pass calibration or there is major drift in the readings.  Revitalizing is used to regenerate the diaphragm in the pH probe. The diaphragm is part of the reference electrode and is necessary for pH measurements.

10.2.2 Perform a ‘cold’ KCl-dip electrode regeneration by heating the Sensor to 60°C / 140°F.

10.2.3 With the Sensor still warm, place saturated KCl solution in the well and let it stand for twenty minutes

10.2.4 Rinse the Sensor with DI water.

10.3.1 The Sensor is very rugged and can withstand harsh environments so any disinfecting products that your lab deems safe to use should be ok to use.

10.3.2 Liquid cannot enter the Sensor so it is recommended to wipe the entire outside and the well with Oosafe or similar disinfectant.

11.1 In order to extend the life of the battery, it is important to store the Sensor in the Sensor Storage/Shut-off Case when not being used for long periods of time.

11.2 Place the sensor with or without buffer A in the Sensor in the Sensor Storage/Shut-off Cas. The orientation of the well is shown on the compartment and must be placed in correctly in order to deactivate the battery.

11.3 If storing the Sensor with Buffer A in it, place the Silicone lid on the Sensor to avoid evaporation and check the Sensor periodically and fill with Buffer A solution when required. This will minimize any drift when using the Sensor.

11.4 To activate the Sensor after being stored, place the Sensor onto the Wireless Charger to activate the Sensor.

The monitor will not power on

Contact Raeyco Lab Equipment Systems Management for support at gopher@raeyco.com or 604.444.0004

Raeyco Lab Equipment Systems Management Ltd.
4428 Lozells Avenue, Suite 205
Burnaby, British Columbia V5A 0C7 Canada